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The contents and changes of endogenous cytokinins and gibberellin in the seeds of ginseng (Panax ginseng C. A. Meyer) during chilling were analyzed with methanol and butanol extracts. Total contents of butanol-soluble free cytokinins, determined with radish cotyledon expansion bioassay, were the highest in the seeds chilled for 2-4 weeks (5.06-8.89 ㎍/g seed) and then markedly decreased during the subsequent 8-16 weeks of chilling treatment. Total gibberellin-like substances, as determined with lettuce hypocotyl bioassay, increased slowly during the stratification period, showing slow increase during the early stage of chilling and then gradually decreased at radicle emergence. The cytokinin contents in the methanol extract of the ginseng seeds, estimated by the radish cotyledon expansion bioassay, decreased with the increase in the duration of chilling of the seeds following seed stratification. The contents of gibberellin-like substances, however, increased with the duration of chilling of moist seeds.
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